Hippocampal Interactions Research Group

led by

Dr. Thibault Cholvin

Using in vivo two-photon calcium imaging in behaving mice, we explore how the brain captures and ensures long-term storage of experiences in cortico-hippocampal networks.

Contact: thibault-cholvin@physiologie.uni-freiburg.de


We conduct research on spatial navigation and episodic memory in behaving mice. Using two-photon in vivo calcium imaging to track the activity of neurons over extended periods of time (multiple days / weeks), we shed light on the properties of the different subregions of the hippocampus (CA1, CA3, DG) as well as their cortical inputs (such as the axonal projections coming from the entorhinal cortex). For this, we combine two-photon imaging and virtual reality to simultaneously capture the activity of hundreds of neurons or axons while the mouse is navigating through virtual environments. Finally, we use the recorded cell or axon activity to decode the animals’ concurrent location and environment, and thus infer the relative involvement of these brain regions in spatial memory.


in vivo two-photon Calcium imaging

We perform two-photon calcium imaging in head-fixed mice performing goal-oriented tasks in familiar or virtual environments.

The animal is standing on an air-supported polystyrene ball, allowing it to navigate through virtual worlds while keeping its actual position constant.

Screens arranged in a hexagonal arc around the mouse and placed ~25 cm away from the head of the animal cover ~260° of the horizontal and ~60° of the vertical visual field of the mouse.

Team members